Figure 3

Curcumin associates with Cdc27. A, DAOY cell lysates were incubated with curcumin-bound sepharose beads and then subjected to SDS-PAGE and immunoblotting with Cdc27. A BubR1 immunoblot was included as control for non-specific binding. B, DAOY cells were incubated with either curcumin or half-curcumin for indicated time points. Cell lysates were separated by SDS-PAGE and immunoblotted for Cdc27. The middle panel shows a longer exposure of the same blot in the upper panel. C, LDH release as a measure of cytotoxicity of curcumin and half-curcumin in DAOY cells treated for 16 h. Data represent mean ± SEM of three independent experiments.