Evidence for different mechanisms of ‘unhooking’ for melphalan and cisplatin-induced DNA interstrand cross-links in vitroand in clinical acquired resistant tumour samples

Spanswick, Victoria J; Lowe, Helen L; Newton, Claire; Bingham, John P; Bagnobianchi, Alessia; Kiakos, Konstantinos; Craddock, Charles; Ledermann, Jonathan A; Hochhauser, Daniel; Hartley, John A

doi:10.1186/1471-2407-12-436

Table 1 Summary of the genes from A549 or RPMI8226 cells whose expression is changed more than two-fold following peak ICL formation by ether melphalan or cisplatin treatment compared to untreated controls

From: Evidence for different mechanisms of ‘unhooking’ for melphalan and cisplatin-induced DNA interstrand cross-links in vitroand in clinical acquired resistant tumour samples

Gene	Fold Regulation
	A549 cells		RPMI8226 cells
	Melphalan (50μM)	Cisplatin (150μM)	Melphalan (50μM)	Cisplatin (150μM)
BTG2	4.86	9.29	−2.06	−1.35
HUS1	2.28	2.49	1.12	−2.77
LIG1	4.43	3.88	1.25	1.13
SESN1	5.71	12.47	1.50	−1.18
TREX1	3.41	5.89	−2.75	−1.27
DMC1	−2.41	−3.97	1.14	−2.16
GADD45A	1.39	2.43	1.64	1.88
PCBP4	1.76	2.66	−4.30	−3.21
PCNA	1.84	2.40	2.58	3.63
XPC	1.88	2.61	−1.60	−1.25
ATM	−1.41	−2.09	1.21	−3.00
ATRX	1.1	−2.15	−1.52	−4.32
BRCA1	−1.24	−2.82	−1.16	−3.44
CHEK2	−1.15	−2.09	−1.24	−2.59
CIDEA	−1.98	−3.33	−16.32	−4.33
GML	−1.98	−3.33	−17.7	−5.77
MAP2K6	−1.61	−4.64	−1.87	−5.92
MNAT1	−1.03	−2.71	−1.23	−3.95
MSH3	1.1	−2.03	1.10	−2.75
RAD51L1	−1.58	−4.94	−3.94	−13.50
SMC1A	−1.51	−2.01	1.10	−1.06
TP73	−1.97	−3.33	−1.57	−3.38
GTSE1	−1.64	−1.69	3.32	2.63
PRKDC	−1.47	−1.97	2.10	1.11
ZAK	1.06	−1.84	3.52	−1.51
RPL13A	−1.34	−1.18	3.10	3.58
DDIT3	1.61	1.92	−7.33	−4.31
IGHMBP2	−1.05	1.14	−2.28	−1.68
IP6K3	1.02	1.94	−16.66	−4.79
NBN	1.3	−1.16	−2.18	−3.11
PMS2L3	−1.56	−1.53	−3.50	−3.24
PPP1R15A	−1.43	−1.06	−11.28	−3.52
RAD17	−1.46	−1.52	−2.21	−2.08
SEMA4A	−1.97	−1.12	−2.17	−3.91
PMS1	−1.29	−1.69	−2.01	−2.92
EXO1	−1.06	1.32	1.60	2.73
FANCG	1.00	−1.06	1.65	2.27
FEN1	−1.03	1.35	1.29	2.03
ATR	−1.54	−1.78	−1.18	−2.24
CHEK1	−1.73	−1.74	−1.08	−13.65
MRE11A	1.19	−1.06	−1.45	−2.78
RAD18	−1.31	−1.61	−1.33	−2.07
RAD50	1.32	−1.12	−1.11	−3.07
XRCC2	1.24	1.23	1.05	−3.66

Data are the mean from three individual drug treated and three control RNA samples in each case. Genes more than two-fold upregulated are shown in red and more than two-fold downregulated in green. For comparison the fold regulation is shown for these genes in both cell lines following both treatments with downregulation shown as negative numbers.

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ISSN: 1471-2407

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