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Figure 6 | BMC Cancer

Figure 6

From: Evidence for different mechanisms of ‘unhooking’ for melphalan and cisplatin-induced DNA interstrand cross-links in vitroand in clinical acquired resistant tumour samples

Figure 6

Real time PCR analysis of the changes in expression of 84 genes involved in DNA damage signalling/repair pathways, following exposure of cells to ICL agent. A: Prior to RNA extraction, A549 cells were treated with melphalan at 50 μM for 1 hour followed by 16 hours post-incubation to allow peak ICL formation. The mean expression from three individual drug treatment repeat samples are compared to three individual non-drug treated control samples. Genes which show increased expression by more than 2-fold compared to control cells following drug treatment are shown in red and those which show decreased expression in green. B: As above, but A549 cells were treated with cisplatin at 150 μM for 1 hour followed by 9 hours post-incubation to allow peak ICL formation.

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