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Figure 5 | BMC Cancer

Figure 5

From: Evidence for different mechanisms of ‘unhooking’ for melphalan and cisplatin-induced DNA interstrand cross-links in vitroand in clinical acquired resistant tumour samples

Figure 5

The roles of homologous recombination and replication in the repair of melphalan and cisplatin-induced ICLs. A: Representative cells showing RAD51 foci following treatment with 5 μM cisplatin followed by post-incubation in drug free medium for the times shown. B: A549 cells were treated with either 2 μM melphalan (red) or 5 μM cisplatin (blue) for 1 hour after which the drug was removed and cells incubated at 37°C in drug-free medium. Samples were taken at different times of post-incubation and RAD51 foci formation determined. Data are the mean ± s.d. from at least three independent experiments. C: RPMI8226 cells were treated with either 2 μM melphalan (red) or 5 μM cisplatin (blue) for 1 hour after which the drug was removed and cells incubated at 37°C in drug-free medium. Samples were taken at different times of post-incubation and RAD51 foci formation determined. Data are the mean ± s.d. from at least three independent experiments. D: Isolated human peripheral blood lymphocytes were treated for 1 hour with either 150 μM cisplatin (blue) or 50 μM melphalan (red) after which the drug was removed and replaced with drug free media. Samples were taken at different times of post-incubation and ICLs measured using the comet assay. Data are the mean from at least two independent experiments.

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