Figure 3

DNA ICL and γ-H2AX foci formation in RPMI8226 cells following treatment with either cisplatin or melphalan. A: Cells were treated for 1 hour with either 150 μM cisplatin (blue) or 50 μM melphalan (red) after which the drug was removed and replaced with drug free media. Samples were taken at different times of post-incubation and ICLs measured using the comet assay. Data are the mean ± s.d. from at least three independent experiments. B: Cells were treated with either 2 μM melphalan (red) or 5 μM cisplatin (blue) for 1 hour after which the drug was removed and cells incubated at 37°C in drug-free medium. Samples were taken at different times of post-incubation and γ-H2AX foci formation determined. Data are the mean ± s.d. from at least three independent experiments.