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Figure 1 | BMC Cancer

Figure 1

From: Evidence for different mechanisms of ‘unhooking’ for melphalan and cisplatin-induced DNA interstrand cross-links in vitroand in clinical acquired resistant tumour samples

Figure 1

Unhooking of DNA ICLs produced by melphalan or cisplatin in clinical samples measured using the single cell gel electrophoresis (comet) assay. A: Plasma cells from two multiple myeloma patients clinically resistant to melphalan. Cells were treated ex vivo for 1 hour with either 150 μM cisplatin or 50 μM melphalan after which the drug was removed and replaced with drug free media. Cells were then incubated for 9 hours (cisplatin, blue) or 16 hours (melphalan, red) post-treatment to allow maximum formation of interstrand cross-links. The level of ICL at this time point was compared to a second sample incubated for a total of 48 hours to obtain the % repair. B: As above but in tumour samples from ovarian cancer patients. In this case the two patients provided samples on initial diagnosis and prior to any platinum-based chemotherapy (Pre) and again following relapse on platinum-based therapy (Post).

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BMC Cancer

ISSN: 1471-2407

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