Effect of genotoxic drugs on AQP3 expression and cell volume. MCF7 (a) or HT29, NP-29 and MDA-MB-468 (c) cells were incubated for 90 minutes (5′-DFUR (DF): 250 μM; gemcitabine (G): 100 nM for MCF7, 250 nM for NP-29 and MDA-MB-468 and 50 μM for HT29; cisplatin (CP): 50 μM) and mRNA was isolated at 24 (a) or 48 hours (a, c). Real-time RT-PCR analysis for AQP3 was performed using GAPDH as endogenous control. Data are calculated as arbitrary units relative to untreated cells (CT) as a reference. Results are the mean ± SE of three to six independent experiments measured in duplicate. At 48 hours after 90 min exposure to the genotoxic drugs, MCF7 (b) or HT29, NP-29 and MDA-MB-468 (d) cells were collected and volumes were measured as cell diameters (μm). Results are the mean ± SE of three to four independent experiments measured in triplicate. Statistical significance was assessed with the Student’s t test (* p < 0.05; ** p < 0.01; *** p < 0.001).