Figure 4

Apoptosis induced by MV-CEA in the human Hep2G and HUH6 cells. The cells were infected with MV-CEA at an MOI of 0.1. Adherent and detached cells were harvested at 24, 48 and 72 hours post infection. (a) The percentage of apoptotic cells was measured by FACS and is shown in this figure. In the L-02 cells, there was no dramatic apoptosis, but the Hep2G cells demonstrated dramatic apoptosis at 72 hours postinfection. (b)The significant difference is significant between the HB and L-02 groups but not between the two HB cell lines. * Group _Hep2G Versus Group _L-02, P < 0.01; **Group _HUH-6 Versus Group _L-02, P < 0.01. (c) We further examined the poly(ADP-ribose) polymerase expression by Western blot and found that the 85-kd cleaved poly(ADP-ribose) polymerase fragment was expressed in the Hep2G and HUH-6 cells at 72 h after infection with MV-CEA. This finding was in agreement with the FACS results.