Genetic association with overall survival of taxane-treated lung cancer patients - a genome-wide association study in human lymphoblastoid cell lines followed by a clinical association study

Table 3 siRNA screening of 11 candidate genes by MTS assay

SNP	Basis for selection				MTS assay
	SNP vs paclitaxel and/or docetaxel IC50 in LCLs	SNP vs survival in SCLC patients	SNP vs survival in NSCLC patients	Gene symbol	SCLC	NSCLC
	(p < 10^-3)	(p < 0.05)	(p < 0.05)		H196	A549
rs7519667	Yes	Yes		CHML	---	Yes
				EXO1	Yes	---
				KMO	Yes	Yes
				OPN3	Yes	---
rs2700868	Yes		Yes	ATP11B	---	---
rs2662411	Yes	Yes		CCT5	Yes	---
				CMBL	Yes	---
rs1106697	Yes	Yes	Yes	RBM33	---	---
rs1778335	Yes	Yes		PIP4K2A	Yes	---
rs17079623	Yes		Yes	TMX3	---	---
rs17304569 & rs7260598	Yes		Yes	ZNF254	---	---

“Yes” under “Basis for selection” indicates individual candidate genes with the p-value listed. For the MTS assay, “Yes” indicates that knockdown of the gene altered taxane cytotoxicity when compared with control siRNA. All of the experiments with significant changes were performed in triplicate and were replicated at least three times.

ISSN: 1471-2407