siRNA screening of candidate genes by MTS assay in lung cancer cell lines. Data are shown for 7 of the 11 candidate genes that were functionally validated in a SCLC cell line, H196, and an NSCLC cell line, A549, by MTS assay after knockdown with specific siRNA pools. (Red) Data for negative control siRNA; (blue) data for specific siRNAs. “Significance” was defined as a gene with a significant change in apparent area under the curve (AUC) in comparison with control siRNA as indicated by the p-values. For those genes with significant change in paclitaxel response, at least three independent experiments were performed in triplicate. Error bar represents standard error of the mean (SEM) for all of the experiments. (A) Candidate gene symbols. (B) qRT-PCR. The y-axis indicates relative gene expression after siRNA knockdown when compared with negative control siRNA. (C) MTS assays. The x-axis indicates the log transformed paclitaxel dose, and the y-axis indicates proportion survival after drug treatment.