Figure 5
From: Hypoxic enhancement of exosome release by breast cancer cells
Cellular and exosomal microRNA expression levels in response to hypoxia. (A) Mean normalised expression levels of MCF7 cellular miRNAs as determined by miRNA-specific Taqman real-time RT-PCR assays in response to 1% O2 for 48 hours and normalised to RNU6B. Expression values are presented relative to normoxic control. (n = 3; ± SEM). (B, C) ExoquickTM precipitants isolated from MCF7 cell culture after 48 hours at 1% O2 were spiked with cel miR-54 and used for RNA extractions. This exosomal RNA was then assayed for miR-16, let7a, miR-210 and cel miR-54 by real-time RT-PCR and normalised to exogenous cel miR-54 (B) or endogenous miR-16 (C) (± SEM). Normoxia n = 4; Hypoxia n = 5. ** corresponds with P value < 0.01.