Exosome isolation by ultracentrifugation and Exoquick.
precipitation from MCF7 conditioned media. (A, B) Transmission electron microscopy and CD63 immunolabelling of MCF7 exosomes isolated by ultracentrifugation (A) and ExoquickTM precipitation (B). (C) Nanoparticle tracking analysis (NTA) of MCF7 exosomes isolated by ultracentrifugation and ExoquickTM precipitation. Data represent the average size distribution profile of n = 4 for each purification method normalised to the total nanoparticle concentrations. Data for each individual sample derive from four different videos and analyses. (D) Immunoblotting of ultracentrifugation pellet for exosomal proteins CD63, TSG101, cofilin and flotillin-1.