Population of the Treg cells in PBMCs. A. CD4+CD25+ T cells were gated after lymphocytes gating and the calculated numbers of CD4+CD25+CD127- T cells in CD4+ T cells were indicated in the representative control, CRA and CRC patient. Percentages of Treg cells are shown in CRA (n = 31), CRC (n = 31), and healthy controls (n = 24) (B), and in patients at early (CRA, n = 19; CRC n = 11) and advanced (CRA, n = 12; CRC n = 20) stages (C). Each datum point represents an individual sample. The horizontal line represents median values for each group.