The population of Th17 cells in PBMCs , NILs and TILs. A. The CD3+CD8- T (CD4+ T) cells were gated after lymphocytes gating. B. Proportions of Th17 cells to CD4+ T subsets from the representative PBMC, NIL and TIL were indicated. Percentages of Th17 cells are shown in CRA (n = 31), CRC (n = 35), and healthy donors (n = 24) (C); in patients at early (CRA, n = 19; CRC n = 14) and advanced (CRA, n = 12; CRC n = 21) stages (D); in PBMCs (n = 20), NILs (n = 20) and TILs (n = 20) from the same CRC patients (E), and in PBMCs and TILs from the same patients at early (n = 9) and advanced (n = 11) stages (F). Each datum point represents an individual sample. The horizontal line represents median values for each group.