Copy Number Amplification of the PIK3CA Gene Is Associated with Poor Prognosis in Non-lymph node metastatic Head and Neck Squamous Cell Carcinoma

Table 2 Primer sequences and annealing temperatures for copy number analysis by qPCR

Gene	Primer sequence	Tm,
PIK3CA	F,5’- GCAAAGGTTGGTCGGTGAA -3’	60
	R,5’- GTGATCTTTGATGTTACTCTGATGCA -3’
KRAS	F,5’- CACCCTAGACAAGCAGCCAATA -3’	60
	R,5’- AAGCCCTGCCGCAAAAA -3’
BRAF	F,5’- CAAGTCACCACAAAAACCTATCGT -3’	60
	R,5- AACTGACTCACCACTGTCCTCTGTT -3’

The copy number changes in KRAS, BRAF and PIK3CA were evaluated by qPCR. DNA from the normal tissue from each sample was used as the control; each gene copy number in the normal tissue was set as 2, and a copy number more than 4 was considered to be a gain.

ISSN: 1471-2407