Skip to main content

Table 1 Primer sequences and annealing temperatures for direct sequencing

From: Copy Number Amplification of the PIK3CA Gene Is Associated with Poor Prognosis in Non-lymph node metastatic Head and Neck Squamous Cell Carcinoma

Gene Exon Primer sequence Tm,
PIK3CA 9 F,5’-TTGCTTTTTCTGTAAATCATCTGTG-3’ 51
   R,5’-CCACAAATATCAATTTACAACCATTG-3’  
  20 F,5’-GGTATTAACATCATTTGCTCCAA-3’ 52
   R,5’-CCTATGCAATCGGTCTTTGC-3’  
KRAS 1 F,5’-CATTACGATACACGTCTGCAGTCAACTGG-3’ 52
   R,5’-GTGAACATCATGGACCCTGACATACTCC-3’  
BRAF 15 F,5’-TCATAATGCTTGCTCTGATAGGA-3’ 52
   R,5-GGCCAAAATTTAATCAGTGGA-3’  
  1. F, forward primer R, reverse primer Tm, annealing temparature.
  2. We used DNA sequencing to detect mutations in the clinical HNSCC specimens. KRAS (exons 1 and 2), BRAF (exon 15) and PIK3CA (exons 9 and 20) were analyzed for mutations.