Figure 4

Simvastatin induces apoptosis in prostate tumor cells in vivo via inhibition of intrinsic cell survival pathway. (A) Western blots showing reduced phosphorylation of Bad, reduced protein expression of Bcl-2 and Bcl-xL, increased release of cytochrome-c from the mitochondria as well as increased protein expression of BimL/BimS, cleaved caspase 9 and cleaved caspase 3 with 14 day simvastatin treatment, compared to saline control in PC3 cell nude mice tumor xenogafts (B) Bar graph showing quantification of the above data by densitometry analysis normalized to β-actin. The data are presented as mean ± SD (n=4 of quadruplicate experiments).