Figure 7
Treatment of HepG2 cell lines with ATO and Sorafenib results in enhanced cytotoxicity and inhibits MAPK activation. HepG2 (A) and LX2 (B) cells were treated with Sorafenib at the indicated concentrations for 24 hr, 48 hr, 72 hr and 96 hr and cell proliferation assessed by MTT assay. For each concentration, percent inhibition values were calculated and data normalized to vehicle control. IC50 values shown in the corresponding tables were determined by non-linear regression in GraphPad Prism. HepG2 (C) and LX2 (D) cells were treated with increasing concentrations of Sorafenib in the absence or presence of ATO (2.5 μM) for 48 h. Inhibition values for ATO alone (2.5 μM) are shown (◊). (E). HepG2 cells were untreated or treated with vehicle, 2.5 μM ATO or 0.1–20 μM sorafenib +/− 2.5 μM ATO as indicated for 24 hrs. Phosphorylated and total MAPK levels were assessed by western blot analysis. GAPDH was utilized as a loading control.