Figure 3

5-FU inhibits HepG2 and LX2 Cell Proliferation at 72 hrs. HepG2 (A) and LX2 (B) cells were treated with 5-FU at the indicated concentrations for 24 and 72 hrs and cell proliferation was assessed by MTT assay. For each concentration, percent inhibition values were calculated and data normalized to vehicle control. IC₅₀ values shown in the corresponding tables were determined by non-linear regression in GraphPad Prism (n = 3 replicates).