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Table 1 Primer and TaqMan probe sequences for qMSP

From: Inhibition of DNA methyltransferase activity and expression by treatment with the pan-deacetylase inhibitor panobinostat in hepatocellular carcinoma cell lines

APC
Forw. 5´-AGTGCGGGTCGGGAAGC-3´
Rev. 5´-AACCACATATCGATCACGTACG-3´
TaqMan-Probe: 5´-FAM-AAAACGCCCTAATCCGCATCCAACG-TAMRA-3´
PCR parameter: Denaturation: 95°C, 10 min. (1 cycle), Amplification: 95°C, 10 sec.; 64°C, 60 sec. (50 cycle); Denaturation: 40°C, 30 sec. (1 cycle)
RASSF1A
Forw. 5´-GCG TTG AAG TCG GGG TTC-3´
Rev. 5´-CCC GTA CTT CGC TAA CTT TAA ACG-3´
TaqMan-Probe: 5´-FAM-ACAAACGCGAACCGAACGAAACCA-TAMRA-3M
PCR parameter: Denaturation: 95°C, 10 min. (1 cycle), Amplification: 95°C, 10 sec.; 63°C, 60 sec. (50 cycle); Denaturation: 40°C, 30 sec. (1 cycle)