Table 1 Primer and TaqMan probe sequences for qMSP

APC

Forw.

5´-AGTGCGGGTCGGGAAGC-3´

Rev.

5´-AACCACATATCGATCACGTACG-3´

TaqMan-Probe:

5´-FAM-AAAACGCCCTAATCCGCATCCAACG-TAMRA-3´

PCR parameter:

Denaturation: 95°C, 10 min. (1 cycle), Amplification: 95°C, 10 sec.; 64°C, 60 sec. (50 cycle); Denaturation: 40°C, 30 sec. (1 cycle)

RASSF1A

Forw.

5´-GCG TTG AAG TCG GGG TTC-3´

Rev.

5´-CCC GTA CTT CGC TAA CTT TAA ACG-3´

TaqMan-Probe:

5´-FAM-ACAAACGCGAACCGAACGAAACCA-TAMRA-3M

PCR parameter:

Denaturation: 95°C, 10 min. (1 cycle), Amplification: 95°C, 10 sec.; 63°C, 60 sec. (50 cycle); Denaturation: 40°C, 30 sec. (1 cycle)