Figure 3From: Inhibition of DNA methyltransferase activity and expression by treatment with the pan-deacetylase inhibitor panobinostat in hepatocellular carcinoma cell lines Regulation of DNA methylation and expression of target genes after panobinostat treatment. DNA methylation of APC (A) and RASSF1A (B) was detected by quantitative methylation-specific PCR in HepG2 and Hep3B cells treated with 0.1 μM panobinostat. Expression of total mRNA for APC (C) and RASSF1A (D) was analyzed using quantitative real-time RT-PCR and normalization to GAPDH content of each sample. Results are mean ± relative error of three independent experiments and are expressed relative to the untreated controls with a set value of 1.0. * P < 0.05 vs. untreated controls.Back to article page