Regulation of DNA methylation and expression of target genes after panobinostat treatment. DNA methylation of APC (A) and RASSF1A (B) was detected by quantitative methylation-specific PCR in HepG2 and Hep3B cells treated with 0.1 μM panobinostat. Expression of total mRNA for APC (C) and RASSF1A (D) was analyzed using quantitative real-time RT-PCR and normalization to GAPDH content of each sample. Results are mean ± relative error of three independent experiments and are expressed relative to the untreated controls with a set value of 1.0. * P < 0.05 vs. untreated controls.