Morphology of cell lines developed from patients 1369, 2295 and 3133 (A-I), including both primary and recurrent cell lines. Passages were between 61 and 67 for each cell line. By the later passages, cell lines had developed into predominantly small epithelial type cells, often aggregated. There was a notable absence of fibroblast cells. Hematoxylin and eosin staining is shown in J-M for solid tumor from patients 1369, 2295 and 3133. Images are taken at x40 magnification. Note that the (t) designation denotes that the primary tumor tissue was investigated.