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Figure 3 | BMC Cancer

Figure 3

From: The T-box transcription factor Brachyury regulates epithelial–mesenchymal transition in association with cancer stem-like cells in adenoid cystic carcinoma cells

Figure 3

Effect of Brachyury shRNA on ACCS-M GFP gene expression related to EMT and CSCs. ACCS-M-sh. control was generated by the transfection of ACCS-M GFP cells with control vector. ACCS-M sh Brachyury and ACCS-M sh Sox2 were generated by the transfection of ACCS-M GFP cells with Brachyury shRNA and Sox2 shRNA, respectively. The mRNA expression levels of the indicated genes in ACCS-M GFP cells and derivatives were quantified by real-time RT-PCR. Each mRNA level was compared with ACCS GFP, and data are shown as relative mRNA levels (ACCS GFP = 1). The expression levels of EMT-related genes (A) and stem cell markers and differentiation markers (B) are shown. Experiments were performed in triplicate, and the number of adhered cells was averaged. Bars indicate the standard deviation.

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BMC Cancer

ISSN: 1471-2407

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