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Figure 2 | BMC Cancer

Figure 2

From: The T-box transcription factor Brachyury regulates epithelial–mesenchymal transition in association with cancer stem-like cells in adenoid cystic carcinoma cells

Figure 2

Analysis of gene expression related to EMT and CSCs by real-time PCR. The mRNA expression levels of the indicated EMT-related genes (A) and embryonic stem cell markers (Nodal, Pax6, Rex1, Lefty, Oct-4, and Nanog) and differentiation markers (mesoderm marker: Brachyury; ectoderm marker: Sox2; endoderm marker: AFP) (B) in ACC cells were quantified by real-time RT-PCR. Each mRNA level was compared between ACCS GFP and ACCS-M GFP, and data are shown as relative mRNA levels to β-actin mRNA. Experiments were performed in triplicate, and the number of adhered cells was averaged. Bars indicate the standard deviation.

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BMC Cancer

ISSN: 1471-2407

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