Table 1 IDH primers
Primer | Â | Â | Tm | Product size |
|---|---|---|---|---|
IDH1 genomic | Forward | CGGTCTTCAGAGAAGCCATT | 59.4 | 113 |
IDH1 genomic | Reverse | GCCAACATGACTTACTTGATCC | 58.6 | Â |
IDH2 genomic | Forward | AACATCCACGCCTAGTCC | 56.3 | 90 |
IDH2 genomic | Reverse | CAGTGGATCCCCTCTCCAC | 60.5 | Â |
IDH1 cDNA | Forward | CGGTCTTCAGAGAAGCCATT | 59.4 | 131 |
IDH1 cDNA | Reverse | AGGCCCAGGAACAACAAAAT | 56.4 | Â |
IDH2 cDNA | Forward | AGTGTGGCTGCAAGTGTGC | 60.0 | 365 |
IDH2 cDNA | Reverse | GAGATGGACTCGTCGGTGTT | 60.1 | Â |