Table 2 Characteristics of microsatellite markers analyzed
Name (locus) | Primer sequence (5’ to 3’) | Unit of repeats | PCR-Tmc | Dye | Size (bp) |
|---|---|---|---|---|---|
TP53-Alu | F…GCA CTT TCC TCA ACT CTA CA | 5 | 55°C | FAM | 382-417 |
|  | R…AAC AGC TCC TTT AAT GGC AG |  |  |  |  |
Mfd41 (D17S261) | F…CAG GTT CTG TCA TAG GAC TA | 2 | 55°C | NED | 153-172 |
|  | R…TTC TGG AAA CCT ACT CCT GA |  |  |  |  |
Mfd28 (D10S89) | F…AAC ACT AGT GAC ATT ATT TTC | 2 | 55°C | FAM | 139-156 |
|  | R…AGC TAG GCC TGA AGG CTT CT |  |  |  |  |
Bat-40 | F…ATT AAC TTC CTA CAC CAC AAC | 1 | 55°C | VIC | 116-132 |
|  | R…GTA GAG CAA GAC CAC CTT G |  |  |  |  |
Bat-26 | F…TGA CTA CTT TTG ACT TCA GCC | 1 | 55°C | FAM | 112-127 |
|  | R…AAC CAT TCA ACA TTT TTA ACC C |  |  |  |  |