Inhibition of PKR does not augment reovirus cytotoxicity in HN cells. A. SIHN 11B, B. PJ41, C. HN3, D. HN5, were incubated with the PKR inhibitor 2-aminopurine (2AP) for 1hr prior to infection with reovirus (MOI 50) using viral stocks at 7.8×108 TCID50/ml. Cell survival was assessed by MTT assay at 96 hours post-infection. Data are representative of 3 independent experiments and error bars are SEMs. E. L929, HN3, HN5, PJ41 and SIHN11b were plated at 1×106/10 cm dish and treated the following day with 2AP (1 mM). After 1 hour incubation with 2AP, reovirus was added at an MOI 50. Cells were incubated for a further 4 hours and then harvested for western analysis with p-EIF2α and γ-tubulin for loading controls.