Figure 6
Reovirus grows at the same rate in EGFR-stimulated or -inhibited SCCHN cells as in untreated cells. A. Cal27, B. SIHN 5B, C. HN3 and D. HN5, were treated overnight with 200 nM epidermal growth factor (EGF) or 400 nM anti-EGFR antibody (ICR62) then infected with reovirus (MOI 10) using viral stocks at 1.9×109 TCID50/ml. Ligands were replaced 2 hrs post infection. Cells and supernatants were harvested at the times indicated for TCID50 titration on L929 cells. Means are from at least 2 independent experiments and error bars represent SEMs.