HN cell lines show varying EGFR expression levels. A. HN cells were stained with anti-EGFR primary antibody (7.5 μg/ml ICR62) then with 20 μg/ml FITC conjugated secondary antibody. Median fluorescence was determined by FACS analysis. Means are from at least 2 independent experiments and error bars represent SEMs. B. Lysates of HN cells indicated were analysed on 10% Precise Protein Gels and subsequently probed for total EGFR, phospho-Tyr1068 EGFR and α-tubulin for loading control. C, D, E. Reovirus IC50 dilutions were assigned rank values (1 = resistant, 15 = sensitive) and were correlated against ranked (C) median fluorescence levels, (D) ranked densitometry values for total EGFR or (E) phospho-tyr1068 EGFR (1 = EGFR low, 9 = EGFR high). R2 = 0.13, -0.43 and −0.67 respectively.