Figure 5

Analysis of lumbar and renal lymph node metastases in PC-3 tumor-bearing mice and of Here, the blood vessel density should be mentioned first (Figure 5A, text labeled in yellow) and lymph nodes second (Figure 5B). (A) Blood vessel density in PC-3 tumor sections, 7 days p.i. For GLV-1h68 and GLV-1h255 blood vessels in infected (vv) and non-infected (ni) areas were counted. Infected areas were determined by expression of the viral marker GFP. For each image (200x, Leica MZ16 FA) blood vessels crossing 5 equidistant lines were counted. (B) Analysis of renal and lumbar lymph node enlargement. PC-3 tumor-bearing mice were injected i.v. with GLV-1h68, GLV-1h255 or PBS. The mice were sacrificed 24 days p.i. and lymph nodes were measured (n = 6–7).