Analysis of the correlation between methylation status and expression of miR-196a. (A) Map of the CpG island position of miR-196a-1. Vertical ticks mark CpG sites. (B) The level of miR-196a expression in 16HBE cells after treatment of 5-aza-dC (0, 2, 5 μM). (C) The methylation status of the CpG island of miR-196a-1 was assessed by bisulfite sequencing before and after 5-aza-dC treatment in 16HBE cells. Open and filled squares denote unmethylated and methylated CpG sites, respectively. Each row represents a single clone. *P < 0.05; **P < 0.01.