qRT-PCR analysis of miR-196a in NSCLC tissues and matched normal tissues. (A) Determining miR-196a expression in NSCLC tissues and its clinical significance. miR-196a was detected in 34 pairs of NSCLC tissues by qRT-PCR. Data are presented as fold-changes in tumor tissues relative to normal tissues. miR-196a expression was significantly higher in patients with a higher pathological stage and lymph-node metastasis. (B) Analysis of miR-196a expression levels in NSCLC cell lines (A549, SPC-A1, NCI-H1650, NCI-H1299 and SK-MES-1) compared with the normal bronchial epithelial cell line (16HBE) by qRT-PCR. (C) Analysis of miR-196a expression following treatment of SPCA1 cells with anti-miR-NC or miR-196a inhibitors by qRT-PCR. (D) Analysis of miR-196a expression following treatment of A549 cells with miR-NC, miR-196a mimics, pCDNA-NC or pCDNA-miR-196a by qRT-PCR. All experiments were performed in biological triplicate with three technical replicates. *P < 0.05, **P < 0.01.