Figure 3
From: Immediate in vivo target-specific cancer cell death after near infrared photoimmunotherapy
In vivo fluorescence and bioluminescence imaging of orthotopic breast tumors in response to Pan-IR700 mediated PIT. (A) Monitoring bioluminescence activity in response to Pan-IR700 mediated PIT (n = 5 each). B: just before NIR light treatment. Bioluminescence signals of tumors in 100 and 150 J/cm2 treated groups showed significant difference with that in control or 50 J/cm2 treated group at all time points examined after PIT (P<0.001). (B) Monitoring bioluminescence activity ratio of treated tumor to internal control (n = 5 each). B: just before NIR light treatment. Bioluminescence signals of tumors in 100 and 150 J/cm2 treated groups showed significant difference with that in control or 50 J/cm2 treated group at all time points examined after PIT (P<0.001). (C) Rapid changes of bioluminescence activity in response to Pan-IR700 mediated PIT (n = 5 each). Before: 1 d before NIR light treatment. (D) IR700 fluorescence and bioluminescence imaging in response to Pan-IR700 mediated PIT. Representative images are shown. Before: just before NIR light treatment, FLI: IR700 fluorescence imaging, WLI: white light imaging, BLI: bioluminescence imaging.