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Figure 4 | BMC Cancer

Figure 4

From: The role of versican G3 domain in regulating breast cancer cell motility including effects on osteoblast cell growth and differentiation in vitro– evaluation towards understanding breast cancer cell bone metastasis

Figure 4

Selective SAPK/JNK inhibitor SP600125 blocks versican G3 enhanced MC3T3-E1 cell apoptosis but doesn’t have effect on G3 inhibited cell growth. (a) The G3- and vector-transfected MC3T3-E1 cells (1 × 103) were inoculated in 96-well culture dishes and cultured in AMDM medium containing 1 ng/ml TGF-β1 with or without 100 nM SP60025 for 7 days. Proliferation assays performed with WST-1 assay. All groups compared with vector control cells, n = 8, * p<0.05, ** p<0.01, analyzed with t-test. (b) The G3- and vector-transfected MC3T3-E1 cells (1 × 105) were cultured in AMDM medium containing 1 ng/ml TGF-β1 with or without 100 nM SP60025 for 24 hours. All samples were lysed and subjected to immunoblotting with antibodies to pSAPK/JNK, GSK-3β (S9P) and β-actin. (c) The vector- and G3-transfected MC3T3-E1 cells were maintained in 10% FBS/AMEM medium with or without 100 nM SP60025 for 21 days. The medium was changed every 3 days. Cells were lysed and processed to ALP ELISA Assay. All groups compared with vector control cells, n = 6, * p<0.05, ** p<0.01, analyzed with t-test. (d) Typical pictures showed ALP staining of the samples.

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