Figure 3

Downregulation of Snail in DU145 and C4-2 cells is associated with increased maspin expression and decrease in cell migratory potential. (A) DU145 cells were transiently transfected with control or Snail siRNA for 3 days followed by PCR and Western blot analysis for Snail and maspin expression. (B) 5X10⁴ DU145 cells that had been treated with control or Snail siRNA was plated in a Boyden chamber and tested for migration on collagen. There was a decrease in migration (mean ± SD, p = 0.071). (C) C4-2 cells stably transduced with non-silencing control (NS) or Snail shRNA lentiviral vectors (E8) displayed decreased Snail and increased maspin expression by PCR and Western blot analysis. (D) 5X10⁴ C4-2 control- or Snail shRNA-expressing cells were tested for migration on collagen using a Boyden chamber. There was a decrease in migration (mean ± SD, p = 0.072) Results are representative of triplicate experiments performed independently.