Induction of ephrin-A1 expression by extracellular S100A4 in NSCLC cell lines. A and B) Fold induction of ephrin-A1 mRNA in NSCLC cell lines treated with 2 μg/ml and 10 μg/ml S100A4 for 6 hours (A) and 24 hours (B) compared to untreated control cells. Bars represent mean fold induction, n = 3, *p < 0.05. (C) Western blot of total cell lysates from A549 cells treated with 2 μg/ml S100A4 for 6 or 24 hours as indicated. Membranes were stained with anti-ephrin-A1, and α-tubulin was used as a loading control.