Fluorescent immuno-histochemical staining of Bax using the HistoRx.
AQUA platform. (A) Representative examples of quantitative fluorescent IHC images for Bax expression in normal OCSE (top panel) and OSCC (two bottom panels). AQUA scores represent the expression level of Bax within the pan-cytokeratin defined epithelial/tumour compartment. DAPI-stained nuclei are depicted in blue, pan-cytokeratin-stained epithelial/tumour cells are depicted in green, and Bax protein expression is depicted in red. (B) Histogram distribution representing Bax expression within OSCC tumour samples. The solid blue line represents median Bax expression in normal OCSE, the broken blue lines represent +/−2 standard deviations from median Bax expression in normal OCSE, and the solid red line represents median Bax expression in OSCC.