Figure 4

ALDH isozyme protein expression in ovarian cell lines. A. Western blot analysis was used to compare the expression of different ALDH isozymes in normal human ovarian surface epithelial (HOSE) cell lines with cancer cell lines of different subtypes, i.e., serous, endometrioid (ENDO), mucinous (MUC) and clear cell (CC). The cell lines were (starting from left): HOSE1-15, HOSE7, HOSE2170, SKOV3, OVCA432, OVCA433, TOV112D, MCAS, RMUGL, RMG1, and OVCA810. Molecular weights are shown on the right. β-actin served as loading control. B. Western blot analysis was used to compare the levels of ALDH1A1 and ALDH7A1 in ovarian cancer cell lines growing as a monolayer (2D) or sphere culture. The cell lines were (starting from left): RMG1, MCAS, RMUGL, OVCA432, SKOV3, and TOV112D. β-actin served as loading control.