Figure 4

Flow cytometric determination of DNA content in shCDCA3- and mock-transfected cells. A) Representative FACS analysis shows that the number of cells in the G1 phase is significantly (*p < 0.05, Mann-Whitney’s U test) increased in shCDCA3-transfected H1 and Sa3 cells. B) Western blot analysis of CDKIs (p21^Cip1 p27^Kip1, p15^INK4B, and p16^INK4A), CDK4, CDK6, Cyclin D1, and Cyclin E shows up-regulation of p21^Cip1, p27^Kip1, p15^INK4B, p16^INK4A, CDK4, and Cyclin D1, and down-regulation of CDK6 and Cyclin E in the CDCA3 knockdown cells