Evaluation of CDCA3 expression in OSCC-derived cell lines. A) Quantification of CDCA3 mRNA expression in OSCC-derived cell lines by qRT-PCR analysis. Significant up-regulation of CDCA3 mRNA is seen in six OSCC-derived cell lines compared with the HNOKs (*p < 0.05, Mann-Whitney’s U test). Data are expressed as the means ± SEM of triplicate results. B) A representative Western blot of CDCA3 protein in OSCC-derived cell lines and HNOKs shows that CDCA3 protein expression is up-regulated in OSCC-derived cell lines compared with HNOKs. Densitometric CDCA3 protein data are normalized to α-tubulin protein levels. The values are expressed as a percentage of the HNOKs.