Fascin overexpression resulted in increase in cell proliferation and tumorigenicity. (A) Histogram showing the total number of colonies formed per plate by the indicated clones after 14 days. Mean and standard deviation of three independent experiments is plotted (p < 0.01). (B) Tumor growth of fascin-overexpressed clones (AW-Fascin-1, AW-Fascin-2) or the vector control (AW-GFP-Cont) was plotted against time interval. (C) Cell proliferation curves of fascin-overexpressed (AW-Fascin-1 and AW-Fascin-2) and vector control (AW-GFP-Cont) cells using MTT assay. The cell number was determined at the times indicated. Each point represents the mean and the standard errors of three independent experiments done in triplicate. (D and E) Western blot analysis of stable fascin-overexpressed (AW-Fascin-1 and AW-Fascin-2) and vector control (AW-GFP-Cont) clones with antibodies to PCNA and phosphorylated forms of Akt, ERK1/2, JNK1/2 and p38. Total Akt, ERK1/2, JNK1/2, p38 and β-actin were used as internal loading controls respectively.