Figure 5

Ephrin-A1 activation attenuates NSCLC cell proliferation. Proliferation of NSCLC cells as measured by WST-1 assay. Plate A: Proliferation in cells either activated with ephrin-A1 or transfected with pcDNA-EFNA1 (pcDNA-EFNA1 is vector containing ephrin-A1 construct). Plate B: Proliferation in cells either silenced with EphA2 siRNA or transfected with pcDNA-EphA2 (pcDNA-EphA2 is vector containing EphA2 construct). Plate C: Proliferation in cdx-2 siRNA transfected cells that were treated with ephrin-A1 ligand or pcDNA-EFNA1 transfected. Data presented is the mean ± SEM of three independent experiments. *p < 0.05 compared to control; $ p < 0.05 compared to ephrin-A1 orpcDNA-EFNA1 alone, # p < 0.001 compared to empty vector or sc-siRNA + ephrinA1, NS is not significant.