Expression of ABCB1 mRNA levels after Wnt1 depletion in CML cell lines. (A) WNT1 siRNA in K562 and Lucena cells. (B) Analysis of ABCB1 mRNA levels after Wnt1 depletion. Total RNA was isolated and used in RT-qPCR analysis to determine changes in ABCB1 mRNA levels after normalization to β-actin expression. All data were presented as fold inductions relative to control group expression (scrambled). Values represent the means of three independent determinations ± s.d.