Western Blot analysis of GSK3 activation. Representative western blot analysis of P-GSK3 from both cell lines protein extracts, with or without LiCl 10 mM treatment. 50 μg of protein extracts were separated SDS-PAGE and probed with anti-P-GSK3 antibody. α-tubulin was used for constitutive expression. For each treatment results, representative of three independent experiments are shown.