Figure 1

Representative staining of VEGF-A (A-C), VEGF-R1 (D-F), and VEGF-R2 (G-I) in IBC and normal breast (A, D, G) samples. With the H-score, IBC49 (B) expressed no epithelial VEGF-A but had low expression (1+) of stromal VEGF-A; IBC65 (C) was also negative for epithelial VEGF-A but had high expression (3+) of stromal VEGF-A (indicated by arrows). IBC samples with low (E, H) and high (F-I) VEGF-R1 (E, F) and VEGF-R2 (H, I) expression, respectively. An angiosarcoma was used as the positive (inset in D) and negative control (isotype-matched antibody; inset in G). Slides were scanned and digitized using the Aperio digital system (Vista, CA).