Expression of WRAP53 protein in distant normal mucosa. (A), primary tumor (B) and lymph node metastasis (C) from the same rectal cancer patient. Frequency of the WRAP53 expression in the whole group of rectal cancer patients (D), non-preoperative radiotherapy group (E) and preoperative radiotherapy group (F). Each bar represents the percentage of cases expressing WRAP53 in each subgroup: distant normal mucosa (DN), adjacent normal mucosa (AN), primary tumor (PT) and lymph node metastatic tumor (MT). The asterisks indicated the significant differences between the DN or AN and PT. The WRAP53 expression in a biopsy taken before radiotherapy (G) and in the corresponding primary tumor after radiotherapy from the same patient (H).