Gene expression profile changes after ectopic expression of ZIC1. (A) The gene expression profiles in pCDNA3.1-ZIC1 or empty pCDNA3.1 vector stable transfectants were analyzed by cDNA microarray in MKN28 cells. Representative genes are illustrated on the right side of the heatmap image using a cut-off >1.5 or < −1.5 fold as a significant difference,. (B) Genes involved in cell cycle, cell proliferation and cell migration according to gene functional analysis (http://www.genecards.org) are presented. Relative fold change is expressed with ratio of pCDNA3.1-ZIC1 versus pCDNA3.1 control. (C) Systemic understanding of pathways for ZIC1 regulation of signaling and target genes in the development and progression of gastric cancer.