Figure 1

Western blot analysis and immunohistochemistry of the surgical specimens obtained from three patients in the current cohort. In the Western blot analysis, Bim was strongly detected in Cases 1 and 3, while Noxa was moderately found in Case 2, and Puma was strongly detected in Case 3. These findings match the results of the immunohistochemical staining analyses. Cell lysates of the Jurkat cells (J) were used as positive controls for Bim and Noxa. HCC2429 (H) was used as the positive control for Puma.