EGCG and C75 induce apoptosis in A549 cells. Induction of caspase activity was confirmed by PARP cleavage. A549 cells were treated with C75 (72 μM) or EGCG (265 μM) for 6, 12 and 24 hours, and equal amounts of lysates were immunoblotted with anti-PARP antibody, which identified the 89 kDa (cleavage product) band. Blots were reproved for β-actin as loading control. Gels shown are representative of those obtained from three independent experiments.