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Figure 1 | BMC Cancer

Figure 1

From: Inhibition of STAT3 signaling and induction of SHP1 mediate antiangiogenic and antitumor activities of ergosterol peroxide in U266 multiple myeloma cells

Figure 1

Ergosterol peroxide (EP) suppresses STAT3 activation in U266 cells. (A) Cells were treated with 25 μM EP for 0, 4, 8, 16 or 24 h. (B) Cells were treated with various concentrations of EP (0, 6.25, 12.5 or 25 μM) for 8 h. (C) SCC4, DU145 and MDA-MB-231 cells were treated with or without EP (25 μM) for 8 h. Whole cell extracts were prepared and subjected to Western blotting to determine level of phospho-STAT3 and STAT3. (D and E) Gel shift mobility assay was performed using nuclear extracts to examine the STAT3-DNA binding activity.

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